Talking about home testing for HIV

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Temperature effects

I have a question about testing temperature. I tested at 13 weeks and did so in my car in a parking lot. It was only about 2 to 4 degrees Celsius outside. I had a bit of trouble getting blood from my cold finger obviously, but the control line did show up and the test was apparently “negative”. I did have my doubts as to whether a faint “Positive” line appeared. So, I tested again at 15 weeks, again under the same conditions (in my car in a parking lot with the temperature also around 4 degrees C). The control line did show up and it DID NOT seem as though a positive line appeared after 20 minutes (though I am always worried it’s faint enough I just haven’t seen it). I do have most all the symptoms of HIV but in each test the result appeared (?) to be negative. Could the temperature have interfered with the test? I did get a control line each time, but perhaps the cold temperatures interfered with the “positive” line showing up? Also how faint of a line are we talking here when it comes to a “Positive” result?  I appreciate your time and feedback. (I apologize if this is a double post, but I didn’t see my last posting listed).

Note from PEBL: This post also appears in the thread where it was originally posted:-


1 Responses

Becky, BioSure UK Ltd
 Work on 20th April 2021

Hi Wondering001, Sorry for not replying sooner, I did not see your reply in the thread, apologies. Our test has been proven to be stable at such temperatures - as long as you received the control line then your test has worked properly and your negative result is reliable. The test line and control line are pre-sprayed onto the strip so it may be possible to almost see the shadow where the test line is positioned on the strip. Your two negative results both outside of the window period are accurate and conclusive - you do not need to retest. I hope this helps. Kindest regards, Becky

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