Not Sure if Blood Sample was enough
I did the biosure test exactly 3 months after a possible exposure and then again 2 months after that (so 5 months after possible encounter) and the result came back negative both times. I’m not sure I performed it correctly because during the first test I didn’t realise the tube to collect blood worked by capillary action so I kind of scooped the blood in, I didn’t know if it was enough so I kept on pressing the tube on the blood spot to take more in (I don’t know if this let out the blood or not and I’m not sure if the inside of the collection space was just simply stained with blood or if the sample was actually still in there in the right amount). I ran the test and it showed the control line, 15 minutes later it was negative (only the control line was showing).
The second time I messed up the lancet and had to cut my finger with a knife to get the blood (not sure if the sample was enough this time as well). I did the test and it came back negative again.
I would like to know how the control line works, i assume it appears when the buffer solution reacts with a protein in human blood, if that’s the case then wouldn’t any amount of blood be able to make it show? (For instance if the walls of the collection space are stained with blood but there is not actually enough of the sample inside for a valid result) and if that’s true wouldn’t that give a false negative since there probably will not be any antibodies in the tiny amount of blood that has been tested?