Talking about home testing for HIV

Tell your story




doubt in reading results

Hi, I took a self test last week at home but i am a bit unsure about the result. Within the first 15 minutest, while the test was in execution in vertical position, only the control line appeared. It came out almost immediately and no other line showed up. However, after the 15 minutes i laid down the test as shown in the manual to read the result and after a couple of minutes a red mark showed up at the bottom of the visible area (way lower than where the test line should be).
A day or two after i looked at the test again and the mark was gone, while the control line was still there.

I wonder, is that mark a line or a just a sample from my blood?
Is the test line supposed to show up within the first 15 minutes of the test execution (while still in vertical position) or is it necessary to lay it down to trigger the line?
Is the test line supposed to show up a that exact position shown in the manual (i guess so) or it can appear at the bottom of the visible area?
Thanks for your help

5 Responses

Francesca Bard, BioSure (UK) Ltd
 Work on 18th January 2019

Hi WORRIED NOW. Please feel free to send any pictures to our WhatsApp number or directly to my email (, I will be happy to confirm your results. The exposure you have described is a very low risk exposure and the symptoms you have stated can be related to lots of things and would not necessarily by HIV. I look forward to hearing from you. Francesca Bard, BioSure (UK) Ltd

Worried Now
 Used on 17th January 2019

Thank you so much for your fast response. Would it be possible for you to check two pictures that I have? Also I had brief oral sex with a man twenty seconds. I had a vomiting bug and weakness in legs and sore In lymph nodes that I still have but not swollen. I had Insti test 8 weeks after possible infection which Was negative. I had a biosure at 8.5 weeks negative. So it would of been 5 weeks and 6 after symptoms that I took the tests. I’ve heard that two weeks after Symptoms it should give correct result. I have taken an INSTI test a week ago which would be 12.5 weeks It was Negative and my last Biosure 13.5 weeks and this is the one I’m confused about. I did think it was Negative but there is a strange curved line after laying it down in box . Today looks like it’s dropped down a little more but just worried because it looks like a line.. even though It’s near blood line below test. Thank you

Francesca Bard, BioSure (UK) Ltd
 Work on 17th January 2019

Hi Worried Now. A positive line can only appear in an exact place. The red at the bottom of the strip is simply migration of the blood sample and buffer solution. This is normal and nothing to worry about. I hope this helps but do let me know if I could assist you further. Kindest regards Francesca Bard, BioSure (UK) Ltd

Worried Now
 Used on 16th January 2019

I have just taken a Biosure test and thought I had a negative result .. clear control line, but when I laid the test down a red curved pink line appeared at the bottom of the test where the blood comes up the strip. It’s not where the test line is . Can you help me please. Worried now

Gary Carpenter, BioSure (UK) Limited
 Work on 15th March 2018

Dear Beebowl Thanks for getting in touch with us. The lines can only appear in the positions shown in the instructions for use. The red mark is a common thing. The mark is caused by the migration of red blood cells from your sample. They can cause a red smudge at bottom of the strip. This is not a line. The test result you describe is negative. The test can run standing up (vertical) or lying down (horizontal). We advise running the test left standing upright. I hope that this answers your questions. Kind regards Gary Carpenter

Post Your Response

  • Tell us your story
  • Label your post

Step 1. Tell your story

Feel free to complete the form below in your own words - write as much or as little as you like! Your comments will be appear anonymously on PEBL.