Talking about home testing for HIV

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Test Line - clarification required

Hi, my test result didn’t show a test line but there was some pink colouring on the corners of where the test line should be. This colouring started appearing after the control line showed and the colouring faded as time passed. 30 min or so after it was all gone. Is that a normal negative result?

For clarity, the control line was there and show up very quickly.

Thanks for the help.


36 Responses

 Used on 18th April 2020

Hi Gary I did the test and it was reading negative but it fell over and a thin wavy line appears below where the positive line would be, there was no bar. My question is can I take this as a negative ? Thanks for your help

 Used on 18th April 2020

I conducted a rapid self test and the purple line appeared on the testing line only - what could be the reason?

Francesca Bard, BioSure (UK) Ltd
 Used on 02nd January 2020

Hi DATEJ, unfortunately I am unable to comment on the test that have been performed and I would recommend contacting the provider of the tests to get answers to your questions. If you would like to retest the BioSURE HIV self test is extremely accurate and very stable (result unlikely to change over time), I will also be able to help you further. I hope this helps and sorry I couldn't assist you further. Kindest regards Francesca Bard, BioSure (UK) Ltd

 Used on 01st January 2020

I used an hiv1/2 antigen/antibody test, after 10minutes it was negative. Then I checked the other day, after like 20hrs. I was surprised to see 1 control dark line and a faint line.

Francesca Bard, BioSure (UK) Ltd
 Used on 12th November 2019

Hi d one, could you possibly email me a picture of your test as I am unsure what has happened with your test and if I can visually see your test I'll be able to help you further. My email address is Kindest regards Francesca Bard, BioSure (UK) Ltd

Francesca Bard, BioSure (UK) Ltd
 Used on 12th November 2019

Hi GENIUS, there are a couple of points in here that I will address. Firstly the test should only be read up to 60 minutes after the barrel was pushed into the buffer pot as although the test is extremely stable there is a possibility of change. Secondly a line can only appear in the exact place (where it has been sprayed) and would appear as a shade of red/pink, a white line could be a reflection. If you would like to send me a picture please do not hesitate and I will be able to look at this test to give you more information ( Kindest regards Francesca Bard, BioSure (UK) Ltd

 Used on 10th November 2019

Hie I need clarity.... my partner kept his negative test kit for almost two months now and a very very faint white line has developed now.... is it that he may be positive? Thank you

D one
 Used on 07th November 2019

I use determine test strip whit unfold buffer and test side appears faint red while the control side appears full red I will appreciate if u will help Gary

Becky Smith, BioSURE UK LTD
 Work on 01st November 2019

HI Sharon, If you still have your test, you can email over a picture of your test and I can confirm your result. My email is Look forward to hearing back from you. Kindest regards, Becky

 Used on 30th October 2019

I did the test on 12 August and first line was light and second line was too dark and I can't I understand my results because that paper is so dark I really need to understand even I started treatment

Gary Carpenter BioSure (UK) Limited
 Work on 15th February 2019

Hi Help!! If you could take a photograph of your test band email it to, I will have a look at the test and come back to you. Kind regards Gary Carpenter BioSure (UK) Limited

 Used on 15th February 2019

I have just done the test and am concerned as there is a dark red line at the top, but a feint blue incomplete line below. I'm not sure what this means and if this counts as a positive result?

Gary Carpenter, BioSure (UK) Limited
 Work on 07th June 2018

Hi Zinger Thanks for contacting BioSure. You haven't clarified which test you did but I'm pretty certain that adding water to top up the amount of blood will pretty much invalidate the result of any HIV test. This would definitely be the case with the BioSURE HIV Self Test. I strongly recommend that you completely ignore the result of the test you have taken and either obtain an approved HIV self test or visit a clinic to get tested. Kind regards Gary Carpenter BioSure (UK) Limited

 Used on 05th June 2018

hi.i did home hiv test so there was no enough blood i put on 2drops of water for an hour or so there was only one strip then the next morning there was a faint line on the test..could i be positive?

Gary Carpenter, BioSure (UK) Limited
 Work on 30th May 2018

Hi Curious Patient Thanks for getting in touch. I don't know that much about the Unigold test so difficult to comment. What I do know, however, is that it is not approved to be used as a self test, so if you have done the test on yourself, you should ignore the result and get one of the approved tests (CE marked) and retest or your should visit a clinic to get a test done. I hope that this helps. Kind regards Gary Carpenter BioSure (UK) Limited

Francesca Bard, BioSure (UK) Ltd
 Work on 30th May 2018

Hi Curious Patient. Unfortunately this is not our test and I am not certain of what a white line would mean. I would also like to let you know that uni gold is NOT an approved HIV self test and is only approved for professional use. I hope this helps. Kindest regards Francesca Bard BioSure (UK) Ltd

Curious patient
 Used on 30th May 2018

I do a HIV test using uni gold the control line is visible while the test line appears as white. Am confused, please what is the meaning of the White line

Gary Carpenter, BioSure (UK) Limited
 Work on 22nd May 2018

Hi Hector Thanks for getting back to me. As far as I know the One Step HIV rapid test is not approved for self-testing for HIV. So my advice would be to ignore that result and either go to a clinic to get tested or if you want to self test then please get a self test that is officially approved for use. If you can let me know where you are based I can let you know what approved tests are available. Thanks. Kind regards Gary

 Used on 22nd May 2018

Hi Gary I used one step anti-HIV (1&2) test.

Gary Carpenter, BioSure (UK) Limited
 Work on 18th May 2018

Hi Hector Thanks for getting in touch. Can you confirm what brand of test you used? Thanks Gary Carpenter BioSure (UK) Limited

 Used on 18th May 2018

I tested using Hiv rapid test and the control line appeared colorful quick and after hours the t est line appeared faint. Does that mean the results are positive?

Gary Carpenter, BioSure (UK) Limited
 Work on 09th February 2018

Hi Hello This is going to be a bit difficult for me to comment on as we do not supply a cassette test as an HIV self test, there aren't any that are approved for use as a self test. So, firstly I would strongly recommend that you do not use an unapproved test. You must find one that has a CE or FDA mark on the box AND on the pouch that contains the test. Secondly, in all probability you will be able to get a valid negative test by just applying buffer to most HIV tests, which is exactly why these sorts of test are not suitable as HIV self tests. The BioSURE HIV Self Test however, has a sample control line, which means that the control will only come up if you have applied a sufficient volume of blood to the test and that the test has run correctly. This is your quality control check. Once the control line on the BioSURE HIV Self Test is triggered you know that you have performed the test procedure correctly and that you have a valid result. I hope that this helps. And please stop using tests that are not regulated. Kind regards Gary Carpenter BioSure (UK) Limited

 Used on 09th February 2018

Hi The hiv casette I use, turns a ghost line when the buffer passes through the T line, it's not pink it's just a a straight line then when the strip turns white in five minutes, that line on T disappears. I would like to make a test to just put the buffer without blood if it's the same. Is there a line at the T that is there from the start? Does the buffer make it visible at one time and disappears when the strip dries up?

 Thinking on 12th January 2018

What makes the control line change colour ?

Gary Carpenter, BioSure (UK) Limited
 Work on 18th December 2017

Hi Secret Thanks for getting in touch with us. The test line and control line can only appear on the test in the positions marked on the results reading part of the instructions for use. It is easiest if you lay the completed test in the test shaped space in the tray. You can then compare your test to the pictures either side. If you could email a picture of your test to me,, I will happily read the test for you and provide clarification of your result. Kind regards Gary Carpenter BioSure (UK) Limited

 Used on 15th December 2017

Hi,I was wondering what happens to the discoloration on the test line and a clear red band on the control that a positive result?cause am confused..the test line was discoloured so I couldn't see any band clearly or would rather say a very faint discolouration like band but saw a clear red band on the control zone

 Used on 07th August 2017

Hi I was just wondering if it is possible for a positive test line to disappear, I tested but forgot to look at the result for over an hour after, the result was negative so would the definitely be negative or could it be possible that it was positive then disappeared. Thanks

Gary Carpenter, BioSure (UK)
 Work on 29th April 2016

Dear Curious No need to thank me! I'm glad that you feel confident in the test. In order to manage HIV at a population level, it's vital that more people access HIV testing. We feel that self-testing offers an important testing option. So it's important that people feel confident in the test. Hopefully our dialogue has managed to answer some other people's questions at the same time. Kind regards Gary

 Used on 28th April 2016

Gary, Thank you for all the explanations and the help (I almost feel like an expert now!) and good luck with the product!

Gary Carpenter, BioSure (UK)
 Work on 28th April 2016

Dear Curious A true test line will not disappear over time, once captured the antibodies for HIV cannot be "washed" away. So if the lines were not permanent, your result is unequivocally negative. Once again hope that helps, and please feel free to ask as many questions as you feel necessary. Kind regards Gary Carpenter BioSure (UK) Limited

 Used on 28th April 2016

Thank you Gary. One last question if I may: From what you said so far, the control line and (real) positive lines dont disappear over time (e.g. 30 min, 1 hour), right? Thank you

Gary Carpenter, BioSure (UK)
 Work on 19th April 2016

Dear Curious You are absolutely right. Over a long period of time the lines can act a bit like a fishing net. The smaller and lighter particles (like the antibodies that the BioSure HIV Self Test detects) move up the strip first. Antibodies trigger the lines. These small non-HIV antibody particles move straight past the test line unaffected and end up being absorbed by the pad at the top of the strip. Much larger and heavier particles, haemoglobin for example, take longer to move up the strip (hours/days for example). These larger particles can get "caught up" in the test line and can discolour the strip where the test line should be. The strip does contain a filter at the bottom which minimises the transfer of these larger particles but some will get through eventually. I hope that helps! Kind regards Gary Carpenter BioSure (UK) Limited

 Used on 18th April 2016

Thanks again Gary, makes sense. By discoloured in this context you mean that a " line" could become visible when it shouldn't, right? Rgds,

Gary Carpenter, BioSure (UK)
 Work on 18th April 2016

Dear Curious There is a possibility that, over time, the test line can become discoloured by haemoglobin that can escape from red blood cells that are present in the sample of blood. Haemoglobin can only escape red blood cells slowly (hours) and it can take a long time (days or weeks) for the discolouration to become visible. When this happens it is not a positive result that has been slow to develop. The BioSure HIV Self Test works by detecting antibodies to HIV. So, to be on the safe side most manufacturers give an upper limit, usually an hour but sometimes as little as 5 minutes (!), for the time that you should read the test. Positive test lines will always start to develop well before this upper limit. Again I hope that this helps... Kind regards Gary Carpenter BioSure (UK)

 Used on 18th April 2016

Thank you Gary, appreciate the quick response. One other question, why do you ask people not to look at the results after an hour? Are they no longer reliable? My partner kept his test for a while after the hour and nothing had changed, the mark was the same. Thanks once more Curious

Gary Carpenter, BioSure (UK)
 Work on 18th April 2016

Dear Curious Thank you for your question. For the result of the test to be positive the test line needs to be complete and permanent. In the example you have given the marks were neither, so your test result is most definitely negative. I hope that this puts your mind at rest. Kind regards Gary Carpenter BioSure (UK)

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